Research Articles
Navigating the Maze: A Researcher's Guide to Validated Microbiome Biomarker Discovery
The translation of microbiome research into clinically actionable biomarkers is fraught with methodological and conceptual challenges.
Beyond the Noise: A Researcher's Guide to Controls for Robust and Reproducible Microbiome Science
This article provides a comprehensive framework for integrating negative and positive controls to enhance the reliability of microbiome research.
Navigating Microbiome Compositional Data Analysis: Overcoming CoDA Challenges for Robust Biomedical Insights
Microbiome sequence data are inherently compositional, with relative abundances constrained by a constant sum, leading to spurious correlations and analytical challenges if ignored.
Mastering Cage Effects and Cohousing: A Researcher's Guide to Reproducible Microbiome Animal Studies
This article provides a comprehensive guide for researchers, scientists, and drug development professionals on managing cage effects and implementing cohousing strategies in animal microbiome studies.
Navigating Microbiome DNA Extraction Kit Batch Effects: A Comprehensive Guide for Robust Research and Reproducibility
Variation between batches of DNA extraction kits is a critical, often overlooked, source of technical bias in microbiome studies.
Power Analysis and Sample Size Calculation in Microbiome Studies: A Practical Guide for Researchers
This article provides a comprehensive guide for researchers and drug development professionals on performing statistically sound power and sample size calculations for microbiome studies.
Navigating Zero-Inflation and Overdispersion: A Statistical Guide for Robust Microbiome Data Analysis
Microbiome data from high-throughput sequencing are characterized by zero-inflation and overdispersion, posing significant challenges for robust statistical analysis in biomedical and clinical research.
Microbiome Data Normalization: A Comprehensive Guide to Rarefaction, Filtering, and Advanced Methods for Robust Analysis
This article provides a comprehensive guide to normalization and filtering for 16S rRNA and shotgun metagenomic data, tailored for researchers and drug development professionals.
A Researcher's Guide to Controlling Contamination in Low Microbial Biomass Microbiome Studies
This article provides a comprehensive framework for researchers and drug development professionals to address the critical challenge of contamination in low microbial biomass microbiome studies.
From Bench to Bedside: The Clinical Translation of the Human Microbiome in Diagnostics and Therapeutics
This article synthesizes the rapid evolution of microbiome science from a research field to a cornerstone of clinical innovation.