Research Articles
A Comprehensive 16S rRNA V3-V4 Amplification Protocol: From Primer Selection to Sequencing Read Validation
This detailed guide provides researchers, scientists, and drug development professionals with a complete workflow for amplifying the 16S rRNA V3-V4 region.
16S rRNA Primer Set Selection: A Comprehensive Comparison of V1-V2 vs V3-V4 Regions for Clinical & Microbiome Research
This article provides a detailed, evidence-based comparison of the 16S rRNA gene V1-V2 and V3-V4 primer sets for microbiome analysis.
16S vs. Shotgun Sequencing: A Comprehensive Guide to Microbial Community Analysis and Method Correlation for Researchers
This article provides a detailed comparative analysis of 16S rRNA gene sequencing and shotgun metagenomics for microbiome research.
16S rRNA vs. Shotgun Metagenomics: A Comprehensive Guide to Choosing the Right Gut Microbiome Analysis Method
This article provides a detailed comparison of 16S rRNA sequencing and shotgun metagenomics for gut microbiome analysis, tailored for researchers and drug development professionals.
16S rRNA vs Shotgun Metagenomics: A Strategic Guide for Biomedical Researchers
This article provides a comprehensive comparison of 16S rRNA gene sequencing and shotgun metagenomics for researchers and drug development professionals.
Controlling Confounding Factors in Microbiome Research: A Strategic Guide for Managing Age, Diet, and Antibiotic Variables
This article provides a comprehensive framework for researchers and drug development professionals to identify, understand, and control for major confounding factors in human microbiome studies.
Controlling False Discoveries in Microbiome Studies: A Comprehensive Guide to FDR Methods for Robust Differential Abundance Analysis
This article provides a comprehensive guide to false discovery rate (FDR) control methods in microbiome differential abundance analysis for researchers and drug development professionals.
Validating Microbiome Biomarkers for Clinical Diagnostics: From Cohort Studies to Clinical Implementation
This comprehensive review examines the critical pathway for validating microbiome-based diagnostic biomarkers across diverse disease contexts.
Validating Microbiome Analysis: From Consensus Standards to Clinical Translation
This article synthesizes current international consensus and best practices for validating microbiome analysis approaches, targeting researchers and drug development professionals.
Bridging the Translational Gap: Establishing Causality Between Animal Models and Human Studies in Microbiome Research
This article provides a comprehensive analysis for researchers and drug development professionals on the critical relationship between animal model findings and human studies in microbiome research.